Transform Yeast  
 

 
  SCOS™ Transformation Kit

For one-step transformation of Saccharomyces cerevisiae cells

The SCOS (Saccharomyces cerevisiae one-step) Transformation Kit provides a simple and fast one-step/onetube method for transforming the yeast, S. cerevisiae with a linear or circular plasmid DNA. Transformation efficiencies with S. cerevisiae will vary based on the yeast strain used, the effi ciency of plasmid integration into the host chromosome, the selection marker used and the transformation procedure chosen. In general,
the entire procedure may be completed in ONE hour, and routinely provides a transformation effi ciency of 103~106 transformants per ug of plasmid DNA.

FEATURES

• HIGH efficiency transformation
• ONE-STEP and ONE-TUBE protocol for transforming S. cerevisiae
• Suitable for cells at any growth phase on plates or broth
• NO MORE preparing competent yeast cells
• Repeatable effi ciency, always reach effi ciency of 103~106/ug DNA

   
 

 
 
Catalog # Description Unit Size Prize Quantity
 YY01-0140    SCOS Yeast Transformation kit. (1 tube/1 Step) Includes:, Carrier DNA, DTT solution, Control Plasmid pYB-L , Control strain TL154.    1 kit    $219.00  
 YY02-0140    SCOS Buffer (mixture of PEG and Cations)    10 ml    $39.00  
 YY03-0140    Carrier DNA (ss DNA to enhance transformation efficiency)    0.5 ml    $49.00  
 YY04-0140    DTT Solution – Reducing agent for improved efficiency    0.5 ml    $39.00  
 YY05-0140    Control plasmid pYB-L (A 2u-ori derived shuttle vector containing Leu2 marker for auxotrophic selection in leu2 - strain)    50 ul (0.2ug/ul)    $169.00  
 YY06-0140    Control Strain TL154 – (Trp1-, leu2- strain, genotype is -alpha, leu2, trp1, gal2, G418s)    200 ul    $169.00  
       
 ACCESSORIES        
       
 YYYT-1040    Lithium Acetate, 1.0 M, pH 7.5 solution    500 ml    $59.95  
 YYYT-1050    TE Buffered Lithium Acetate, pH 7.5 sterile solution    500 ml    $59.95  
 YYYT-1060    Sorbitol, 1.0 M sterile solution    500 ml    $59.95  
 YYYT-1070    Sorbitol-Tris-CaCl2Solution, pH 7.5 sterile solution    500 ml    $49.95  
 YYYT-1080    Tris-CaCl2Solution, pH 7.5 sterile solution    500 ml    $49.95  
       
 
 ECOS Value Pack: 10 LB Agar + Antibiotic Plates , Plating Beads and 1 Liter of Liquid Media of Choice. SCOS Value Pack: YPD Agar Plates, YPD Broth, Plating Beads, and drop out Media  


 


 

 

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