X-Gal: 5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside

Enzyme Substrates for LacZ screening. Ideal for cloning in Bacteria, Yeasts, Mammalian cells and insect cells. Perform Blue-White Assays and see the difference.

CytoBLUE™, EZ-Bluez™, & Firozeh™ are (patent pending) room temperature stable components of the beta gal detection systems. They have a longer shelf life than general X-Gal, IPTG and X-alpha-Gal solution. The proprietary aqueous based solvent protects the reagents from rapid breakdown at room temperature and induces brighter colorimetric reactions. They are superior in performance, ready to use, solutions, exclusively produced and tested for all cloning and reporter assays. They are least toxic, do not require –20°C freezing, and are stable at RT for extended periods. With these substrates there is no need to re-incubate or refrigerate to enhance color. NO DMSO, NO DMF, NO Waiting, Shelf Life > 1 year at 40°C.

Cloning in E. coli is almost synonymous with Blue-White Assays. X-Gal is among the most commonly used substrate that is used for studying LacZ expression. EZ-Bluez™ is a pre-made X-Gal solution that eliminates the need to thaw, or freeze the substrate. A ready to use solution with a concentration of 10 mg/mL is ideal for all beta-gal screening assays. Firozeh™ is an X-Gal/IPTG solution which is designed to induce as LacZ expression in the presence of (lacIq) lac repressor. Although these substrates are insoluble in water they are usually dissolved in hazardous solvents such as DMSO or DMF. Both these solvents are unstable, breakdown after repeated freeze-thaw, and take longer to absorb on agar as an overlay. Furthermore IPTG induction protocols often suggests storing agar plates in refrigerator to enhance the blue color.

X-Gal: 5-Bromo-4-chloro-3-indolyl-beta-D-galactopyranoside

Analytical Specifications: C14H15BrClNO6, CAS RN: [7240-90-6], Molecular Weight: 408.63 g/mol, Assay: Greater than 99% (HPLC), Solution: Clear (2% in DMF), Biological Activity: Blue color with Lambda, pUC and M13, Elemental Analysis: Agrees with theoretical values, Thin Layer Chromatogram: Single Spot, Melting point: 230°C (d)